Микология и фитопатология, 2023, T. 57, № 5, стр. 321-331
The Peculiarities of the Mycobiota Formation on the Saint Petersburg Stone Monuments Based on Metagenomics and Cultural Data
K. V. Sazanova 1, 2, 3, *, M. S. Zelenskaya 2, **, O. A. Pavlova 4, ***, D. Yu. Vlasov 1, 2, ****
1 Komarov Botanical Institute of the Russian Academy of Sciences
197022 St. Petersburg, Russia
2 St. Petersburg State University
199034 St. Petersburg, Russia
3 Archive of the Russian Academy of Sciences, St. Petersburg Branch
196084 St. Petersburg, Russia
4 Beagle LTD Company
192289 St. Petersburg, Russia
* E-mail: ksazanova@binran.ru
** E-mail: marsz@yandex.ru
*** E-mail: beagle07@bk.ru
**** E-mail: dmitry.vlasov@mail.ru
Поступила в редакцию 15.04.2023
После доработки 20.05.2023
Принята к публикации 23.05.2023
- EDN: YECIMP
- DOI: 10.31857/S0026364823050070
Полные тексты статей выпуска доступны в ознакомительном режиме только авторизованным пользователям.
Аннотация
Fungi play an important role in colonization and biodeterioration of stone monuments in the open air. This study significantly complements the data on fungal diversity in lithobiotic communities through the use of metagenomic analysis. It was shown that the mycobiota of tombstones in the historical center of St. Petersburg has a complex structure. There are different complexes of species, which have various origins and ways of getting to the monuments. The complex of dominant fungi in SABs on marble and granite in St. Petersburg is formed by dark-colored micromycetes. At the species level, the absolute dominant in all samples was the Knufia karalitana according to metagenomic data and Aureobasidium pullulans according to cultural data. The use of two methodological approaches indicates the expediency of combining culture-based and molecular genetics methods, which make it possible to obtain the most more complete picture of the formation of lithobiotic communities. The season and the type of biofilm have a key importance for the abundance and diversity of micromycetes on stone surface. It was shown that type of rock has a minimal importance for the fungal diversity on stone monument.
Полные тексты статей выпуска доступны в ознакомительном режиме только авторизованным пользователям.
Список литературы
Burford E., Kierans M., Gadd M. Geomycology: fungi in mineral substrata. Mycologist. 2003. V. 17. P. 98–07. https://doi.org/10.1017/S0269-915X(03)00311-2
Cappitelli F., Principi P., Pedrazzani R. et al. Bacterial and fungal deterioration of the Milan cathedral marble treated with protective synthetic resins. Sci. Total Environ. 2007. V. 385. P. 172–181. https://doi.org/10.1016/j.scitotenv.2007.06.022
Dakal T.C., Arora P.K. Evaluation of potential of molecular and physical techniques in studying biodeterioration. Rev. Environ. Sci. BioTechnol. 2012. V. 11. P. 71–104. https://doi.org/10.1007/s11157-012-9264-0
De Leo F., Antonelli F., Pietrini A.M. et al. Study of the euendolithic activity of black meristematic fungi isolated from a marble statue in the Quirinale Palace’s Gardens in Rome, Italy. Facies. 2019. V. 65. https://doi.org/10.1007/s10347-019-0564-5
De Leo F., Marchetta A., Urzì C. Black fungi on stone-built heritage: Current knowledge and future outlook. Appl. Sci. 2022. V. 12. (3969). https://doi.org/10.3390/app12083969
De Leo F., Urzi C. Microfungi from deteriorated materials of cultural heritage. In: Fungi from different substrates. Misra J.K. et al. (eds). CRC Press, Taylor and Francis group, N.Y., 2015, pp. 144–158.
Farooq M., Hassan M., Hassan M. et al. Mycobial deterioration of stone monuments of Dhamarajika, Taxila. J. Microbiol. Experimentation. 2015. V. 2 (1). P. 29–33. https://doi.org/10.15406/jmen.2015.02.00036
Fungal sequencing and classification with the ITS metagenomics protocol. 2018. https://www.illumina.com/content/dam/illumina-marketing/documents/products/ appnotes/its metagenomics-app-note-1270-2018-001-web.pdf.
Fungal metagenomic sequencing. demonstrated protocol. 2019. https://support.illumina.com/content/dam/illuminasupport/documents/documentation/chemistry_ documentation/metagenomic/fungal-metagenomic-demonstrated-protocol-1000000064940-01.pdf.
González J.M., Sáiz-Jiménez C. Application of molecular nucleic acid-based techniques for the study of microbial communities in monuments and artworks. Int. Microbiol. 2005. V. 8. P. 189–194.
Gorbushina A. Life on the rocks. Environ. Microbiol. 2007. V. 9. P. 1613–1631. https://doi.org/10.1111/j.1462-2920.2007.01301.x.
Gorbushina A.A., Vlasov D.Yu., Lyalikova N.N. et al. Microbial communities on the monuments of Moscow and St. Petersburg: Biodiversity and trophic relations. Microbiology. 2002. V. 71 (3). P. 350–356.
Isola D., Bartoli F., Meloni P. et al. Black fungi and stone heritage conservation: ecological and metabolic assays for evaluating colonization potential and responses to traditional biocides. Appl. Sci. 2022. V. 12 (2038). https://doi.org/10.3390/app12042038
Isola D., Zucconi L., Onofri G.S. et al. Extremotolerant rock inhabiting black fungi from Italian monumental sites. Fungal Diversity. 2016. V. 76. P. 75–96. https://doi.org/10.1007/s13225-015-0342-9
Kirtsideli I.Yu., Kazanova A.V., Lazarev P.A. et al. Development of rock-inhabiting microfungi on artificial (synthetic) marble sculptures in the Summer Garden (St. Petersburg). In: Biogenic-abiogenic interactions in natural and anthropogenic systems / O.V. Frank-Kamenetskaya etc. (eds). Springer, N.Y. etc., 2016, pp. 393–401.
Kurakov A.V., Somova N.G., Ivanovskii R.N. Micromycetes populating limestone and red brick surfaces of the Novodevichii convent masonry. Microbiology. 1999. V. 68 (2). P. 273–282 (in Russ.).
Liu X., Koestler R.J., Warscheid T. Microbial deterioration and sustainable conservation of stone monuments and buildings. Nat. Sustain. 2020. V. 3. P. 991–1004. https://doi.org/10.1038/s41893-020-00602-5
Mang S.M., Scrano L., Camele I. Preliminary studies on fungal contamination of two rupestrian churches from Matera (Southern Italy). Sustainability. 2020. V. 12 (6988). https://doi.org/10.3390/su12176988
Mihajlovski A., Seyer D., Benamara H. et al. An overview of techniques for the characterization and quantification of microbial colonization on stone monuments. Ann. Microbiol. 2015. V. 65. 1243–1255. https://doi.org/10.1007/s13213-014-0956-2
Onofri S., Zucconi L., Isola D. et al. Rock-inhabiting fungi and their role in the deterioration of stone monuments in the Mediterranean area. Plant Biosyst. 2014. V. 148 (2). P. 384–391. https://doi.org/10.1080/11263504.2013.877533
Ortega-Morales B.O., Narváez-Zapata J., Reyes-Estebanez M. et al. Bioweathering potential of cultivable fungi associated with semi-arid surface microhabitats of Mayan buildings. Front. Microbiol. 2016. V. 7 (201). https://doi.org/10.3389/fmicb.2016.00201
Paiva D.S., Fernandes L., Trovão J. et al. Uncovering the fungal diversity colonizing limestone walls of a forgotten monument in the central region of Portugal by high-throughput sequencing and culture-based methods. Appl. Sci. 2022. V. 12. P. 10650. https://doi.org/10.3390/app122010650
Salvadori O., Municchia A.C. The role of fungi and lichens in the biodeterioration of stone monuments. The Open Conference Proceeding Journal. 2015. N 6 (Suppl 1: M 4). P. 70–82. https://doi.org/10.2174/2210289201607020039
Santo A.P., Cuzman O.A., Petrocchi D. et al. Black on white: microbial growth darkens the external marble of Flo-rence Cathedral. Appl. Sci. 2021. 11. 6163. https://doi.org/10.3390/app11136163
Sazanova K.V., Zelenskaya M.S., Vlasov A.D. et al. Microorganisms in superficial deposits on the stone monuments in St. Petersburg. Microorganisms. 2022. V. 10. P. 316. https://doi.org/10.3390/microorganisms10020316
Sazanova K.V., Zelenskaya M.S., Bobir S.Yu. et al. Micromycetes in biofilms on stone monuments of Saint Petersburg. Mikologiya i fitopatologiya. 2020. V. 54 (5). P. 329–339. https://doi.org/10.31857/S0026364820050104
Suihko M.L., Alakomi H.L., Gorbushina A. et al. Characterization of aerobic bacterial and fungal microbiota on surfaces of historic Scottish monuments. Syst. Appl. Microbiol. 2007. V. 30. P. 494–508. https://doi.org/10.1016/j.syapm.2007.05.001
Trovãoa J., Portugala A., Soaresa F. et al. Fungal diversity and distribution across distinct biodeterioration phenomena in limestone walls of the old cathedral of Coimbra, UNESCO World Heritage Site. International Biodeterioration and Biodegradation. 2019. V. 142. P. 91–102. https://doi.org/10.1016/j.ibiod.2019.05.008
Villa F., Stewart P.S., Klapper I. et al. Subaerial biofilms on outdoor stone monuments: Changing the perspective toward an ecological framework. BioSci. 2016. V. 66. P. 285–294. https://doi.org/10.1093/biosci/biw006.
Vladimirov I.A., Matveva T.V., Lutova L.A. Real-time RCR for agrobacteria distribution study. Galanika, SPb., 2014.
Vlasov D.Yu., Zelenskaya M.S., Frank-Kamenetskaya O.V. Micromycetes on marble monuments of Alexander-Nevskaya lavra Museum necropolises (Saint Petersburg). Mikologiya i fitopatologiya. 2002. V. 36 (3). P. 7–10 (in Russ.).
Wollenzien U., de Hoog G.S., Krumbein W.E. et al. On the isolation of microcolonial fungi occurring on and in marble and other calcareous rocks. Sci. Total Environ. 1995. № 167. P. 287–294.
Zhang Y., Su M., Wu F. et al. Diversity and composition of culturable microorganisms and their biodeterioration potentials in the Sandstone of Beishiku Temple, China. Microorganisms. 2023. V. 11. P. 429. https://doi.org/10.3390/ microorganisms11020429
Власов Д.Ю., Зеленская М.С., Франк-Каменецкая О.В. (Vlasov et al.) Микромицеты на мраморных памятниках музейных некрополей Александро-Невской Лавры (Санкт-Петербург) // Микология и фитопатология, 2002. Т. 36. № 3. С. 7–10.
Горбушина А.А., Ляликова Н.Н., Власов Д.Ю. и др. (Gorbushina et al.) Микробные сообщества на мраморных памятниках Санкт-Петербурга и Москвы: видовой состав (разнообразие) и трофические взаимодействия // Микробиология. 2002. Т. 71. № 3. С. 409–417.
Кураков А.В., Сомова Н.Г., Ивановский Р.Н. (Kurakov et al.) Микромицеты – обитатели поверхности белокаменных и кирпичных сооружений Новодевичьего монастыря // Микробиология. 1999. Т. 68. № 2. С. 273–282.
Сазанова К.В., Зеленская М.С., Бобир С.Ю. и др. (Sazanova et al.) Микромицеты в биопленках на каменных памятниках Санкт-Петербурга // Микология и фитопатология. 2020. Т. 54. № 5. С. 329–339.
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