Молекулярная биология, 2023, T. 57, № 3, стр. 387-410
Улучшение культурных растений при помощи системы CRISPR/Cas: новые гены-мишени
Ю. В. Ухатова a, *, М. В. Ерастенкова a, Е. С. Коршикова a, Е. А. Крылова a, А. С. Михайлова a, Т. В. Семилет a, Н. Г. Тихонова a, Н. А. Швачко a, Е. К. Хлесткина a
a Федеральный исследовательский центр Всероссийский институт генетических ресурсов растений
им. Н.И. Вавилова
190000 Санкт-Петербург, Россия
* E-mail: sci_secretary@vir.nw.ru
Поступила в редакцию 04.05.2022
После доработки 23.09.2022
Принята к публикации 07.10.2022
- EDN: CHWGLZ
- DOI: 10.31857/S0026898423030151
Полные тексты статей выпуска доступны в ознакомительном режиме только авторизованным пользователям.
Аннотация
Успехи геномного редактирования сельскохозяйственных культур с использованием системы CRISPR/Cas в большой степени зависят от правильного выбора генов-мишеней, направленные изменения в которых позволят повысить урожайность, улучшить качество растительного сырья и устойчивость к биотическим и абиотическим стрессирующим факторам. В настоящей работе систематизированы и каталогизированы сведения о генах-мишенях, использованных для улучшения культурных растений. В последнем систематическом обзоре рассмотрены статьи, индексируемые в базе данных Scopus, опубликованные на 17.08.2019 г. В нашей работе охвачен период с 18.08.2019 по 15.03.2022 гг. Поиск по заданному алгоритму позволил выявить 2090 статей, среди которых только 685 содержат результаты редактирования генов 28 видов культурных растений (поиск проведен по 56 культурам). В значительной части этих публикаций рассмотрено либо редактирование генов-мишеней, проведенное ранее в аналогичных работах, либо исследования относились к сфере обратной генетики, и только 136 статей содержат данные о редактировании новых генов-мишеней, модификация которых направлена на улучшение селекционно значимых признаков растений. Всего за весь период применения системы CRISPR/Cas с целью улучшения селекционно значимых свойств редактированию были подвергнуты 287 генов-мишеней культурных растений. В настоящем обзоре представлен подробный анализ редактирования новых генов-мишеней. Чаще всего целью этих работ было повышение урожайности и устойчивости растений к болезням, а также улучшение свойств растительного сырья. Отмечено, удалось ли на момент публикации получить стабильные трансформанты, применялось ли редактирование к немодельным сортам. Существенно расширен спектр модифицированных сортов ряда культур, в частности, пшеницы, риса, сои, томата, картофеля, рапса, винограда, кукурузы. В подавляющем большинстве случаев редактирующие конструкции доставляли с использованием агробактериальной трансформации, реже – биобаллистики, трансфекции протопластов и гаплоиндукторов. Желаемого изменения признаков чаще всего удавалось достичь при помощи нокаута генов. В отдельных случаях осуществляли нокдаун и замены нуклеотидов в гене-мишени. Для получения нуклеотидных замен в генах культурных растений все чаще используют редактирование отдельных оснований (base-editing) и технологию поиска и замены (prime-editing). Появление удобной системы редактирования CRISPR/Cas способствовало развитию молекулярной частной генетики многих культурных видов растений.
Полные тексты статей выпуска доступны в ознакомительном режиме только авторизованным пользователям.
Список литературы
Korotkova A.M., Gerasimova S.V., Shumny V.K., Khlestkina E.K. (2017) Crop genes modified using the CRISPR/Cas system. Russ. J. Genet. 7(8), 822–832.https://doi.org/10.1134/S2079059717050124)
Короткова А.М., Герасимова С.В., Хлесткина Е.К. (2019) Текущие достижения в области модификации генов культурных растений с использованием системы CRISPR/Cas. Вавил. журн. генет. cелекции. 23(1), 29–37.
Zegeye W.A., Chen D., Islam M., Wang H., Riaz A., Rani M.H., Hussain K., Liu Q., Zhan X., Cheng S., Cao L., Zhang Y. (2022) OsFBK4, a novel GA insensitive gene positively regulates plant height in rice (Oryza sativa L.). Ecol. Genet. Genom. 23. https://doi.org/10.1016/j.egg.2022.100115
Wu Q., Liu Y., Huang J. (2022) CRISPR-Cas9 mediated mutation in OsPUB43 improves grain length and weight in rice by promoting cell proliferation in spikelet hull. Int. J. Mol. Sci. 23(4), 2347. https://doi.org/10.3390/ijms23042347
Kim C.Y., Park J.Y., Choi G., Kim S., Vo K.T.X., Jeon J.S., Kang S., Lee Y.H. (2022) A rice gene encoding glycosyl hydrolase plays contrasting roles in immunity depending on the type of pathogens. Mol. Plant Pathol. 23(3), 400–416. https://doi.org/10.1111/mpp.13167
Li B., Du X., Fei Y., Wang F., Xu Y., LI X., Li W., Chen Z., Fan F., Wang J., Tao Y., Jiang Y., Zhu Q.‑H., Yang J. (2021) Efficient breeding of early-maturing rice cultivar by editing PHYC via CRISPR/Cas9. Rice. 14, 86. https://doi.org/10.1186/s12284-021-00527-3
Hu J., Huang L., Chen G., Liu H., Zhang Y., Zhang R., Zhang S., Liu J., Hu Q., Hu F., Wang W., Ding Y. (2021) The elite alleles of OsSPL4 regulate grain size and increase grain yield in rice. Rice. 14, 90. https://doi.org/10.1186/s12284-021-00531-7
Duy P.N., Lan D.T., Thu H.P., Thanh H.N., Pham N.P., Auguy F., Thi B.T.H., Manh T.B., Cunnac S., Pham X.H. (2021) Improved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter. PLoS One. 16(9), e0255470. https://doi.org/10.1371/journal.pone.0255470
Dong S., Dong X., Han X., Zhang F., Zhu Y., Xin X., Wang Y., Hu Y., Yuan D., Wang J., Huang Z., Niu F., Hu Z., Yan P., Cao L., He H., Fu J., Xin Y., Tan Y., Mao B., Zhao B., Yang J., Yuan L., Luo X. (2021) OsPDCD5 negatively regulates plant architecture and grain yield in rice. Proc. Natl. Acad. Sci. USA. 118(29), e2018799118. https://doi.org/10.1073/pnas.2018799118
Nurhayati, Ardie S.W., Santoso T.J., Sudarsono. (2021) CRISPR/Cas9-mediated genome editing in rice cv. IPB3S results in a semi-dwarf phenotypic mutant. Biodiversitas J. Biol. Diversity. 22(9), 3792–3800. https://doi.org/10.13057/biodiv/d220924
Tao H., Shi X., He F., Wang D., Xiao N., Fang H., Wang R., Zhang F., Wang M., Li A., Liu X., Wang G.L., Ning Y. (2021) Engineering broad-spectrum disease-resistant rice by editing multiple susceptibility genes. J. Integr. Plant Biol. 63(9), 1639–1648. https://doi.org/10.1111/jipb.13145
Nawaz G., Usman B., Peng H., Zhao N., Yuan R., Liu Y., Li R. (2020) Knockout of Pi21 by CRISPR/-Cas9 and iTRAQ-based proteomic analysis of mutants revealed new insights into M. oryzae resistance in elite rice line. Genes. 11, 735. https://doi.org/10.3390/genes11070735
Zheng S., Ye C., Lu J., Liufu J., Lin L., Dong Z., Li J., Zhuang C. (2021) Improving the rice photosynthetic efficiency and yield by editing OsHXK1 via CRISPR/Cas9 system. Int. J. Mol. Sci. 22, 9554. https://doi.org/10.3390/ijms22179554
Zeng Y., Wen J., Zhao W., Wang Q., Huang W. (2020) Rational improvement of rice yield and cold tolerance by editing the three genes OsPIN5b, GS3, and O-sMYB30 with the CRISPR-Cas9 system. Front. Plant Sci. 10. 1663. https://doi.org/10.3389/fpls.2019.01663
Wu M., Liu H., Lin Y., Chen J., Fu Y., Luo J., Zhang Z., Liang K., Chen S., Wang F. (2020) In-frame and frame-shift editing of the Ehd1 gene to develop japonica rice with prolonged basic vegetative growth periods. Front. Plant Sci. 11, 307. https://doi.org/10.3389/fpls.2020.00307
Honma Y., Adhikari P.B., Kuwata K., Kagenishi T., Yokawa K., Notaguchi M., Kurotani K., Toda E., Bessho-Uehara K., Liu X., Zhu S., Wu X., Kasahara R.D. (2020) High-quality sugar production by osgcs1 rice. Commun. Biol. 3, 617. https://doi.org/10.1038/s42003-020-01329-x
Usman B., Nawaz G., Zhao N., Liu Y., Li R. (2020) Generation of high yielding and fragrant rice (Oryza sativa L.) lines by CRISPR/Cas9 targeted mutagenesis of three homoeologs of cytochrome P450 gene family and OsBADH2 and transcriptome and proteome profiling of revealed changes triggered by mutations. Plants. 9, 788. https://doi.org/10.3390/plants9060788
Wang G., Wang C., Lu G., Wang W., Mao G., Habben J.E., Song C., Wang J., Chen J., Gao Y., Liu J., Greene T.W. (2020) Knockouts of a late flowering gene via CRISPR–Cas9 confer early maturity in rice at multiple field locations. Plant. Mol. Biol. 104, 137–150. https://doi.org/10.1007/s11103-020-01031-w
Zhang A., Liu Y., Wang F., Li T., Chen Z., Kong D., Bi J., Zhang F., Luo X., Wang J., Tang J., Yu X., Liu G., Luo L. (2019) Enhanced rice salinity tolerance via CRISPR/Cas9-targeted mutagenesis of the OsRR22 gene. Mol. Breeding. 39(3). 47. https://doi.org/10.1007/s11032-019-0954-y
Kim Y.A., Moon H., Park C.J. (2019) CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae. Rice. 12, 67. https://doi.org/10.1186/s12284-019-0325-7
Hu X., Cui Y., Dong G., Feng A., Wang D., Zhao C., Zhang Y., Hu J., Zeng D., Guo L., Qian Q. (2019) Using CRISPR-Cas9 to generate semi-dwarf rice lines in elite landraces. Sci. Rep. 9, 19096. https://doi.org/10.1038/s41598-019-55757-9
Yang Q., Ding J., Feng X., Zhong X., Lan J., Tang H., Harwood W., Li Z., Guzmán C., Xu Q., Zhang Y., Jiang Y., Qi P., Deng M., Ma J., Wang J., Chen G., Lan X., Wei Y., Zheng Y., Jiang Q. (2022). Editing of the starch synthase IIa gene led to transcriptomic and metabolomic changes and high amylose starch in barley. Carbohydrate Polymers. 285, 119238. https://doi.org/10.1016/j.carbpol.2022.119238
Galli M., Martiny E., Imani J., Kumar N., Koch A., Steinbrenner J., Kogel K.H. (2022) CRISPR/SpCas9-mediated double knockout of barley microrchidia MORC1 and MORC6a reveals their strong involvement in plant immunity, transcriptional gene silencing and plant growth. Plant Biotechnol. J. 20(1), 89–102. https://doi.org/10.1111/pbi.13697
Lee J.H., Won H.J., Tran P.H.N., Lee S.-Mi, Je H.-Y.K., Jung H. (2021) Improving lignocellulosic biofuel production by CRISPR/Cas9-mediated lignin modification in barley. GCB Bioenergy. 13, 742–752. https://doi.org/10.1111/gcbb.12808
Gerasimova S.V., Hertig C., Korotkova A.M., Kolosovskaya E.V., Otto I., Hiekel S., Kochetov A.V., Khlestkina E.K., Kumlehn J. (2020) Conversion of hulled into naked barley by Cas endonuclease-mediated knockout of the NUD gene. BMC Plant Biol. 20, 255. https://doi.org/10.1186/s12870-020-02454-9
Герасимова С.В., Короткова А.М., Хертинг K., Хикель C., Хоффи Р., Будхагатапалли Н., Отто И., Хензель Г., Шумный В.К., Кочетов А.В., Кумлен Й., Хлесткина Е.К. (2018) Применение РНК-направленной нуклеазы Cas9 для сайт-специфической модификации генома в протопластах cибирского сорта ячменя с высокой способностью к регенерации. Вавил. журн. генет. селекции. 22(8), 1033–1039. https://doi.org/10.18699/VJ18.447
Holubova K., Hensel G., Vojta P., Tarkowski P., Bergougnoux V., Galuszka P. (2018) Modification of barley plant productivity through regulation of cytokinin content by reverse-genetics approaches. Front. Plant. Sci. 9, 1676. https://doi.org/10.3389/fpls.2018.01676
Gasparis S., Przyborowski M., Kała M., Nadolska-Orczyk A. (2019) Knockout of the HvCKX1 or HvCKX3 gene in barley (Hordeum vulgare L.) by RNA-guided Cas9 nuclease affects the regulation of cytokinin metabolism and root morphology. Cells. 8(8), 782. https://doi.org/10.3390/cells8080782
Ibrahim S., Saleem B., Rehman N., Zafar A.S., Naeem M.K., Khan M.R. (2021) CRISPR/Cas9 mediated disruption of inositol pentakisphosphate 2-kinase 1 (TaIPK1) reduces phytic acid and improves iron and zinc accumulation in wheat grains. J. Adv. Res. 1–9. https://doi.org/10.1016/j.jare.2021.07.006
Guo M., Wang Q., Zong Y., Nian J., Li H., Li J., Wang T., Gao C., Zuo J. (2021) Genetic manipulations of TaARE1 boost nitrogen utilization and grain yield in wheat. J. Genet. Genomics. 48(10), 950‒953. https://doi.org/10.1016/j.jgg.2021.07.003
Zhang S., Zhang R., Gao J., Gu T., Song G., Li W., Li D., Li Y., Li G. (2019) Highly efficient and heritable targeted mutagenesis in wheat via the Agrobacterium tumefaciens-mediated CRISPR/Cas9 system. Int. J. Mol. Sci. 20(17), 4257. https://doi.org/10.3390/ijms20174257
Raffan S., Sparks C., Huttly A., Hyde L., Martignago D., Mead A., Hanley S.J., Wilkinson P.A., Barker G., Edwards K.J., Curtis T.Y., Usher S., Kosik O., Halford N.G. (2021) Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2. Plant Biotechnol. J. 19(8), 1602–1613. https://doi.org/10.1111/pbi.13573
Hahn F., Loures S.L., Sparks C.A., Kanyuka K., Nekrasov V. (2021) Efficient CRISPR/Cas-mediated targeted mutagenesis in spring and winter wheat varieties. Plants. 10, 1481.https://doi.org/10.3390/plants10071481
Li J., Jiao G., Sun Y., Chen J., Zhong Y., Yan L., Jiang D., Ma., Xia, L. (2021) Modification of starch composition, structure and properties through editing of T-aSBEIIa in both winter and spring wheat varieties by CRISPR/Cas9. Plant Biotechnol. J. 19, 937–951. https://doi.org/10.1111/pbi.13519
Tang H., Liu H., Zhou Y., Liu H., Du L., Wang K., Ye X. (2020) Fertility recovery of wheat male sterility controlled by Ms2 using CRISPR/Cas9. Plant Biotechnol. J. 19(2), 224‒226. https://doi.org/10.1111/pbi.13482
Budhagatapalli N., Halbach T., Hiekel S., Büchner H., Müller A.E., Kumlehn J. (2020) Site-directed mutagenesis in bread and durum wheat via pollination by Cas9/guide RNA-transgenic maize used as haploidy inducer. Plant Biotechnol. J. 18(12), 2376–2378.
Wang W., Pan Q., Tian B., He F., Chen Y., Bai G., Akhunov E. (2019) Gene editing of the wheat homologs of TONNEAU1-recruiting motif encoding gene affects grain shape and weight in wheat. Plant J. 100, 251‒264. https://doi.org/10.1111/tpj.14440
Abe F., Haque E., Hisano H., TanakaT., Kamiya Y., Mikami M., Sato K. (2019) Genome-edited triple-recessive mutation alters seed dormancy in wheat. Cell Rep. 28(5), 1362–1369.e4. https://doi.org/10.1016/j.celrep.2019.06.090
Zhang Z., Hua L., Gupta A., Tricoli D., Edwards K.J., Yang B., Li W. (2019) Development of an Agrobacterium-delivered CRISPR/Cas9 system for wheat genome editing. Plant Biotechnol. J. 17, 1623‒1635. https://doi.org/10.1111/pbi.13088
Brauer E.K., Balcerzak M., Rocheleau H., Leung W., Schernthaner J., Subramaniam R., Ouellet T. (2020) Genome editing of a deoxynivalenol-induced transcription factor confers resistance to fusarium graminearum in wheat. Mol. Plant Microbe Interact. 33(3), 553–560. https://doi.org/10.1094/MPMI-11-19-0332-R
Camerlengo F., Frittelli A., Sparks C., Doherty A., Martignago D., Larre C., Lupi R., Sestili F., Masci S. (2020) CRISPR-Cas9 multiplex editing of the α-amylase/trypsin inhibitor genes to reduce allergen proteins in durum wheat. Front. Sustain. Food Syst. 4, 104. https://doi.org/10.3389/fsufs.2020.00104
Guan H., Chen X., Wang K., Liu X., Zhang D., Li Y., Song Y., Shi Y., Wang T., Li C., Li Y. (2022) Genetic variation in ZmPAT7 contributes to tassel branch number in maize. Int. J. Mol. Sci. 23(5), 2586. https://doi.org/10.3390/ijms23052586
Li Y., Lin Z., Yue Y. Zhao H., Fei X., Lizhu E., Liu C., Chen S., Lai J., Song W. (2021) Loss-of-function alleles of ZmPLD3 cause haploid induction in maize. Nat. Plants. 7, 1579–1588. https://doi.org/10.1038/s41477-021-01037-2
Wang Y., Liu X., Zheng X., Wang W., Yin X., Liu H., Ma C., Niu X., Zhu J.K., Wang F. (2021) Creation of aromatic maize by CRISPR/Cas. J. Integr. Plant. Biol. 63(9), 1664‒1670. https://doi.org/10.1111/jipb.13105
Gao L., Yang G., Li Y., Sun Y., Xu R., Chen Y., Wang Z., Xing J., Zhang Y. (2021) A Kelch-repeat superfamily gene, ZmNL4, controls leaf width in maize (Zea mays L.). Plant J. 107(3), 817‒830. https://doi.org/10.1111/tpj.15348
Liu L., Gallagher J., Arevalo E.D., Chen R., Skopelitis T., Wu Q., Bartlett M., Jackson D. (2021) Enhancing grain-yield-related traits by CRISPR-Cas9 promoter editing of maize CLE genes. Nat. Plants. 7(3), 287‒294. https://doi.org/10.1038/s41477-021-00858-5
Li Q., Wu G., Zhao Y., Wang B., Zhao B., Kong D., Wei H., Chen C., Wang H. (2020) CRISPR/Cas9-mediated knockout and overexpression studies reveal a role of maize phytochrome C in regulating flowering time and plant height. Plant Biotechnol. J. 18(12), 2520‒2532. https://doi.org/10.1111/pbi.13429
Jiang Y.Y., Chai Y.P., Lu M.H., Han X.L., Lin Q., Zhang Y., Zhang Q., Zhou Y., Wang X.C., Gao C., Chen Q.J. (2020) Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize. Genome Biol. 21(1), 257. https://doi.org/10.1186/s13059-020-02170-5
Li Y., Zhu J., Wu H., Liu C., Huang C., Lan J., Zhao Y., Xie C. (2020) Precise base editing of non-allelic acetolactate synthase genes confers sulfonylurea herbicide resistance in maize. Crop J. 8, 449‒456. https://doi.org/10.1016/j.cj.2019.10.001
Qi X., Wu H., Jiang H., Zhu J., Huang C., Zhang X., Liu C., Cheng B. (2020) Conversion of a normal maize hybrid into a waxy version using in vivo CRISPR/Cas9 targeted mutation activity. Crop J. 8, 440‒448. https://doi.org/10.1016/j.cj.2020.01.006
Zhao X., Jayarathna S., Turesson H., Fält A., Nestor G., González M.N., Olsson N., Beganovic M., Hofvander P., Andersson R., Andersson M. (2021) Amylose starch with no detectable branching developed through DNA-free CRISPR-Cas9 mediated mutagenesis of two starch branching enzymes in potato. Sci. Rep. 11, 4311. https://doi.org/10.1038/s41598-021-83462-z
Tuncel A., Corbin K.R., Ahn-Jarvis J., Harris S., Hawkins E., Smedley M.A., Harwood W., Warren F.J., Patron N.J., Smith A.M. (2019) Cas9-mediated mutagenesis of potato starch-branching enzymes generates a range of tuber starch phenotypes. Plant Biotechnol. J. 17, 2259‒2271. https://doi.org/10.1111/pbi.13137
Takeuchi A., Ohnuma M., Teramura H., Asano K., Noda T., Kusano H., Tamura K., Shimada H. (2021) Creation of a potato mutant lacking the starch branching enzyme gene StSBE3 that was generated by genome editing using the CRISPR/dMac3-Cas9 system. Plant Biotechnol. (Tokyo). 38(3), 345‒353. https://doi.org/10.5511/plantbiotechnology.21.0727a
Zheng Z., Ye G., Zhou Y., Pu X., Su W., Wang J. (2021) Editing sterol side chain reductase 2 gene (StSSR2) via CRISPR/Cas9 reduces the total steroidal glycoalkaloids in potato. All Life. 14(1), 401‒413. https://doi.org/10.1080/26895293.2021.1925358
Gonzalez M.N., Massa G.A., Andersson M., Turesson H., Olsson N., Fält A.-S., Storani L., Oneto D.C.A., Hofvander P., Feingold S.E. (2020) Reduced enzymatic browning in potato tubers by specific editing of a polyphenol oxidase gene via ribonucleoprotein complexes delivery of the CRISPR/Cas9 system. Front. Plant Sci. 10, 1649. https://doi.org/10.3389/fpls.2019.01649
Kieu N.P., Lenman M., Wang E.S., Petersen B.L., Andreasson E. (2021) Mutations introduced in susceptibility genes through CRISPR/Cas9 genome editing confer increased late blight resistance in potatoes. Sci. Rep. 11(1), 4487. https://doi.org/10.1038/s41598-021-83972-w
Osakabe Y., Liang Z., Ren C., Nishitani C., Osakabe K., Wada M., Komori S., Malnoy M., Velasco R., Poli M., Jung M.-H., Koo O.-J., Viola R., Kanchiswamy C.N. (2018) CRISPR–Cas9-mediated genome editing in apple and grapevine. Nat. Protoc. 13(12), 2844‒2863. https://doi.org/10.1038/s41596-018-0067-9
Scintilla S., Salvagnin U., Giacomelli L., Zeilmaker T., Malnoy M.A., van der Voort J.R., Moser C. (2021) Regeneration of plants from DNA-free edited grapevine protoplasts.https://doi.org/10.1101/2021.07.16.452503
Olivares F., Loyola R., Olmedo B., Miccono M.D.L.Á., Aguirre C., Vergara R., Riquelme D., Madrid G., Plantat P., Mora R., Espinoza D., Prieto H. (2021) CRISPR/Cas9 targeted editing of genes associated with fungal susceptibility in Vitis vinifera L. cv. Thompson Seedless using geminivirus-derived replicons. Front. Plant Sci. 12, 791030. https://doi.org/10.3389/fpls.2021.791030
Wan D.Y., Guo Y., Cheng Y., Hu Y., Xiao S., Wang Y., Wen Y.Q. (2020) CRISPR/Cas9-mediated mutagenesis of VvMLO3 results in enhanced resistance to powdery mildew in grapevine (Vitis vinifera). Horticulture Res. 7, 116. https://doi.org/10.1038/s41438-020-0339-8
Yang L., Guo Y., Hu Y., Wen Y. (2020) CRISPR/Cas9-mediated mutagenesis of VviEDR2 results in enhanced resistance to powdery mildew in grapevine (Vitis vinifera). Acta Horticulturae Sinica. 47(4), 623‒634. https://doi.org/10.16420/j.issn.0513-353x.2019-0660
Li M., Jiao Y., Wang Y., Zhang N., Wang B., Liu R., Yin X., Xu Y., Liu G. (2020) CRISPR/Cas9-mediated VvPR4b editing decreases downy mildew resistance in grapevine (Vitis vinifera L.). Hortic. Res. 7(1), 149. https://doi.org/10.1038/s41438-020-00371-4
Sunitha S., Rock C.D. (2020) CRISPR/Cas9-mediated targeted mutagenesis of TAS4 and MYBA7 loci in grapevine rootstock 101-14. Transgenic Res. 29(3), 355–367. https://doi.org/10.1007/s11248-020-00196-w
Ren C., Guo Y., Kong J., Lecourieux F., Dai Z., Li S., Liang Z. (2020) Knockout of VvCCD8 gene in grapevine affects shoot branching. BMC Plant Biol. 20(1), 47. https://doi.org/10.1186/s12870-020-2263-3
Tripathi J.N., Ntui, V.O., Shah T., Tripathi L. (2021) CRISPR/Cas9-mediated editing of DMR6 orthologue in banana (Musa spp.) confers enhanced resistance to bacterial disease. Plant Biotechnol. J. 1(7), 1291‒1293. https://doi.org/10.1111/pbi.13614
Hu C., Sheng O., Deng G., He W., Dong T., Yang Q., Dou T., Li C., Gao H., Liu S., Yi G., Bi F. (2021) CRISPR/Cas9-mediated genome editing of MaACO1 (aminocyclopropane-1-carboxylate oxidase 1) promotes the shelf life of banana fruit. Plant Biotechnol. J. 19(4), 654‒656. https://doi.org/10.1111/pbi.13534
Varkonyi-Gasic E., Wang T., Cooney J., Jeon S., Voogd C., Douglas M.J., Pilkington S.M., Akagi T., Allan A.C. (2021) Shy Girl, a kiwifruit suppressor of feminization, restricts gynoecium development via regulation of cytokinin metabolism and signalling. New Phytol. 230, 1461‒1475. https://doi.org/10.1111/nph.17234
Pompili V., Costa L. D., Piazza S., Pindo M., Malnoy M. (2019) Reduced fire blight susceptibility in apple cultivars using a high-efficiency CRISPR/Cas9-FLP/FRT-based gene editing system. Plant Biotechnol. J. 18(3), 845‒858. https://doi.org/10.1111/pbi.13253
Omori M., Yamane H., Li K., Matsuzaki R., Ebihara S., Li T., Tao R. (2020) Expressional analysis of FT and CEN genes in a continuously flowering highbush blueberry “Blue Muffin”. Acta Hortic. 1280, 197‒201. https://doi.org/10.17660/ActaHortic.2020.1280.27
Jia H., Wang Y., Su H., Huan X., Wang N. (2022) LbCa-s12a-D156R efficiently edits LOB1 effector binding elements to generate canker-resistant citrus plants. Cells. 11(3), 315. https://doi.org/10.3390/cells11030315
Zhou J., Wang G., Liu Z. (2018) Efficient genome editing of wild strawberry genes, vector development and validation. Plant Biotechnol. J. 16(11), 1868‒1877. https://doi.org/10.1111/pbi.12922
Feng J., Dai C., Luo H., Han Y., Liu Z., Kang C. (2019) Reporter gene expression reveals precise auxin synthesis sites during fruit and root development in wild strawberry. J. Exp. Botany. 70(2), 563‒574. https://doi.org/10.1093/jxb/ery384
Bottero E., Gomez C., Stritzler M., Tajima H., Frare R., Pascuan C., Blumwald E., Ayub N., Soto G. (2022) Generation of a multi‑herbicide‑tolerant alfalfa by using base editing. Plant Cell Rep. 41, 493–495. https://doi.org/10.1007/s00299-021-02827-w
Zhang Z., Wang J., Kuang H., Hou Z., Gong P., Bai M., Zhou S., Yao X., Song S., Yan L., Guan Y. (2022) Elimination of an unfavorable allele conferring pod shattering in an elite soybean cultivar by CRISPR/Cas9. ABIOTECH. https://doi.org/10.1007/s42994-022-00071-8
Chen X., Yang S., Zhang Y., Zhu X., Yang X., Zhang C., Li H., Feng X. (2021) Generation of male-sterile soybean lines with the CRISPR/Cas9 system. Crop J. 9(6), 1270–1277. https://doi.org/10.1016/j.cj.2021.05.003
Wang T., Xun H., Wang W., Ding X, Tian H., Hussain S., Dong Q., Li Y., Cheng Y., Wang C., Lin R., Li G., Qian X., Pang J., Feng X., Dong Y., Liu B., Wang S. (2021) Mutation of GmAITR genes by CRISPR/Cas9 genome editing results in enhanced salinity stress tolerance in soybean. Front. Plant Sci. 12, 779598. https://doi.org/10.3389/fpls.2021.779598
Cai Z., Xian P., Cheng Y., Ma Q., Lian T., Nian H., Ge L. (2021) CRISPR/Cas9-mediated gene editing of GmJAGGED1 increased yield in the low-latitude soybean variety Huachun 6. Plant Biotechnol. J. 19(10), 1898‒1900. https://doi.org/10.1111/pbi.13673
Li Z., Cheng Q., Gan Z., Hou Z., Zhang Y., Li Y., Li H., Nan H., Yang C., Chen L., Lu S., Shi W., Chen L., Wang Y., Fang C., Kong L., Su T., Li S., Kou K., Wang L., Kong F., Liu B., Dong L. (2021) Multiplex CRISPR/Cas9-mediated knockout of soybean LNK2 advances flowering time. Crop J. 9(4), 767‒776. https://doi.org/10.1016/j.cj.2020.09.005
Ma J., Sun S., Whelan J., Shou H. (2021) CRISPR/Cas9-mediated knockout of GmFATB1 significantly reduced the amount of saturated fatty acids in soybean seeds. Int. J. Mol. Sci. 22, 3877. https://doi.org/10.3390/ijms22083877
Nguyen C.X., Paddock K.J., Zhang Z., Stacey M.G. (2021) GmKIX8-1 regulates organ size in soybean and is the causative gene for the major seed weight QTL qSw17-1. New Phytol. 229, 920–934. https://doi.org/10.1111/nph.16928
Adachi K., Hirose A., Kanazashi Y., Hibara M., Hirata T., Mikami M., Endo M., Hirose S., Maruyama N., Ishimoto M., Abe J., Yamada T. (2021) Site-directed mutagenesis by biolistic transformation efficiently generates inheritable mutations in a targeted locus in soybean somatic embryos and transgene-free descendants in the T1 generation. Transgenic Res. 30, 77–89. https://doi.org/10.1007/s11248-020-00229-4
Le H., Nguyen N.H., Ta D.T., Le T.N.T., Bui T.P., Le N.T., Nguyen C.X., Rolletschek H., Stacey G., Stacey M.G., Pham N.B., Do P.T., Chu H.H. (2020) CRISPR/Cas9-mediated knockout of galactinol synthase-encoding genes reduces raffinose family oligosaccharide levels in soybean seeds. Front. Plant Sci. 11, 612942. https://doi.org/10.3389/fpls.2020.612942
Sugano S., Hirose A., Kanazashi Y., Adachi K., Hibara M., Itoh T., Mikami M., Endo M., Hirose S., Maruyama N., Abe J., Yamada T. (2020) Simultaneous induction of mutant alleles of two allergenic genes in soybean by using site-directed mutagenesis. BMC Plant Biol. 20, 513. https://doi.org/10.1186/s12870-020-02708-6
Chen L., Nan H., Kong L., Yue L., Yang H., Zhao Q., Fang C., Li H., Cheng Q., Lu S., Kong F., Liu B., Dong L. (2020) Soybean AP1 homologs control flowering time and plant height. J. Integr. Plant Biol. 62, 1868‒1879. https://doi.org/10.1111/jipb.12988
Wang L., Sun S., Wu T., Liu L., Sun X., Cai Y., Li J., Jia H., Yuan S., Chen L., Jiang B., Wu C., Hou W., Han T. (2020) Natural variation and CRISPR/Cas9-mediated mutation in GmPRR37 affect photoperiodic flowering and contribute to regional adaptation of soybean. Plant Biotechnol. J. 18, 1869‒1881. https://doi.org/10.1111/pbi.13346
Zhang P., Du H., Wang J., Pu Y., Yang C., Yan R., Yang H., Cheng H., Yu D. (2020) Multiplex CRISPR/-Cas9-mediated metabolic engineering increases soya bean isoflavone content and resistance to soya bean mosaic virus. Plant Biotechnol. J. 18(6), 1384‒1395. https://doi.org/10.1111/pbi.13302
Hou Z.H., Wu Y., Cheng Q., Gan Z.R., Liu B.H. (2019) Creation of high oleic acid soybean mutation plants by CRISPR/Cas9. Acta Agronomica Sinica (China). 45(6), 839‒847.
Do P.T., Nguyen C.X., Bui H.T., Tran L.T.N. Stacey G., Gillman J.D., Zhang Z.J. Stacey M.J. (2019) Demonstration of highly efficient dual gRNA CRISPR/Cas9 editing of the homeologous GmFAD2–1A and GmFAD2–1B genes to yield a high oleic, low linoleic and α-linolenic acid phenotype in soybean. BMC Plant Biol. 19, 311. https://doi.org/10.1186/s12870-019-1906-8
Wu N., Lu Q., Wang P., Zhang Q., Zhang J., Qu J., Wang N. (2020) Construction and analysis of G-mFAD2-1A and GmFAD2-2A soybean fatty acid desaturase mutants based on CRISPR/Cas9 technology. Int. J. Mol. Sci. 21, 1104. https://doi.org/10.3390/ijms21031104
Cai Y., Wang L., Chen L., W T., Liu L., Sun S., Wu C., Yao W., Jiang B., Yuan S., Han T., Hou W. (2020) Mutagenesis of GmFT2a and GmFT5a mediated by CRISPR/Cas9 contributes for expanding the regional adaptability of soybean. Plant Biotechnol. J. 18, 298‒309. https://doi.org/10.1111/pbi.13199
Han J., Guo B., Guo Y., Zhang B., Wang X., Qiu L.-J. (2019) Creation of early flowering germplasm of soybean by CRISPR/Cas9 technology. Front. Plant Sci. 10, 1446. https://doi.org/10.3389/fpls.2019.01446
Bao A., Chen H., Chen L., Chen S., Hao Q., Guo W., Qiu D., Shan Z., Yang Z., Yuan S., Zhang C., Zhang X., Liu B., Kong F., Li X., Zhou X., Trna L.-S.P., Cao D. (2019) CRISPR/Cas9-mediated targeted mutagenesis of GmSPL9 genes alters plant architecture in soybean. BMC Plant Biol. 19, 131. https://doi.org/10.1186/s12870-019-1746-6
Badhan S., Ball A.S., Mantri N. (2021) First report of CRISPR/Cas9 mediated DNA-free editing of 4CL and RVE7 genes in chickpea protoplasts. Int. J. Mol. Sci. 22, 396. https://doi.org/10.3390/ijms22010396
Dai X., Han H., Huang W., Zhao L., Song M., Cao X., Liu C., Niu X., Lang Z., Ma C., Xie H. (2022) Generating novel male sterile tomatoes by editing respiratory burst oxidase homolog genes. Front. Plant Sci. 12, 817101. https://doi.org/10.3389/fpls.2021.817101
Kawaguchi K., Takei‑Hoshi R., Yoshikawa I., Nishida K., Kobayashi M., Kusano M., Lu Y., Ariizumi T., Ezura H., Otagaki S., Matsumoto S., Shiratake K. (2021) Functional disruption of cell wall invertase inhibitor by genome editing increases sugar content of tomato fruit without decrease fruit weight. Sci. Rep. 11(1), 21534.
Wang B., Li N., Huang S., Hu J., Wang Q., Tang Y., Yang T., Asmutola P., Wang J., Yu Q. (2021) Enhanced soluble sugar content in tomato fruit using CRISPR/Cas9-mediated SlINVINH1 and SlVPE5 gene editing. Peer. J. 9. e12478. https://doi.org/10.7717/peerj.12478
Bari V.K., Nassar J.A., Aly R. (2021) CRISPR/Cas9 mediated mutagenesis of MORE AXILLARY GROWTH 1 in tomato confers resistance to root parasitic weed Phelipanche aegyptiaca. Sci. Rep. 11, 3905. https://doi.org/10.1038/s41598-021-82897-8
Hanika K., Schipper D., Chinnappa S., Oortwijn M., Schouten H.J., Thomma B.P.H.J., Bai Y. (2021) Impairment of tomato WAT1 enhances resistance to vascular wilt fungi despite severe growth defects. Front. Plant Sci. 12, 721674. https://doi.org/10.3389/fpls.2021.721674
Liu H., Lihong Liu, Liang D., Zhang M., Jia C., Qi M., Liu Y., Shao Z., Meng F., Hu S., Yin Y., Li C., Wang Q. (2021) SlBES1 promotes tomato fruit softening through transcriptional inhibition of PMEU1. Science. 24(8), 102926. https://doi.org/10.1016/j.isci.2021.102926
Thomazella D.P.D.T., Seong K., Mackelprang R., Dahlbeck D., Geng Y., Gill U.S., Qi T., Pham J., Giuseppe P., Lee C.Y., Ortega A., Cho M., Hutton S.F., Staskawicz B. (2021) Loss of function of a DMR6 ortholog in tomato confers broad-spectrum disease resistance, Proc. Natl Acad. Sci. USA. 118(27), e2026152118. https://doi.org/10.1073/pnas.2026152118
Tran M.T., Doan D.T.H., Kim J., Song Y.J., Sung Y.W., Das S., Kim E.J, Son G.H., Kim S.H., Van Vu T., Kim J.Y. (2021) CRISPR/Cas9-based precise excision of SlHyPRP1 domain(s) to obtain salt stress-tolerant tomato. Plant Cell Rep. 40(6), 999–1011. https://doi.org/10.1007/s00299-020-02622-z
Liu J., Wang S., Wang H., Luo B., Cai Y., Li X., Zhang Y., Wang X. (2021) Rapid generation of tomato male-sterile lines with a marker use for hybrid seed production by CRISPR/Cas9 system. Mol. Breed. 41(3), 25. https://doi.org/10.1007/s11032-021-01215-2
Atarashi H., Jayasinghe W.H., Kwon J., Kim H., Taninaka Y., Igarashi M., Ito K., Yamada T., Masuta C., Nakahara K.S. (2020) Artificially edited alleles of the eukaryotic translation initiation factor 4E1 gene differentially reduce susceptibility to cucumber mosaic virus and potato virus Y in tomato. Front. Microbiol. 11, 564310. https://doi.org/10.3389/fmicb.2020.564310
Yoon Y.J., Venkatesh J., Lee J.H., Kim J., Lee H.E., Kim D.S., Kang B.C. (2020) Genome editing of EIF4E1 in tomato confers resistance to pepper mottle virus. Front. Plant Sci. 11, 1‒11. https://doi.org/10.3389/fpls.2020.01098/full
Kuroiwa K., Thenault C., Nogue F., Perrot L., Maziera M., Galloisa J.L. (2022) CRISPR-based knock-out of EIF4E2 in a cherry tomato background successfully recapitulates resistance to pepper veinal mottle virus. Plant Sci. 316, 111160. https://doi.org/10.1016/j.plantsci.2021.111160
Jung Y.J., Kim D.H., Lee H.J., Nam K.H., Bae S., Nou I.S., Cho Y.-G., Kim M.K., Kang K.K. (2020) Knockout of SlMS10 gene (Solyc02g079810) encoding bHLH transcription factor using CRISPR/Cas9 system confers male sterility phenotype in tomato. Plants. 9(9), 1189. https://doi.org/10.3390/plants9091189
Illouz-Eliaz N., Nissan I., Nir I., Ramon U., Shohat H., Weiss D. (2020) Mutations in the tomato gibberellin receptors suppress xylem proliferation and reduce water loss under water-deficit conditions. J. Exp. Botany. 71(12), 3603–3612. https://doi.org/10.1093/jxb/eraa137
Santillán Martínez M.I., Bracuto V., Koseoglou E., Appiano M., Jacobsen E., Visser R.G.F., Wolters A.M.A., Bai Y. (2020) CRISPR/Cas9-targeted mutagenesis of the tomato susceptibility gene PMR4 for resistance against powdery mildew. BMC Plant Biol. 20, 284. https://doi.org/10.1186/s12870-020-02497-y
Faal G.P., Farsi M., Seifi A., Kakhki A.M. (2020) Virus-induced CRISPR-Cas9 system improved resistance against tomato yellow leaf curl virus. Mol. Biol. Rep. 47(5), 3369–3376. https://doi.org/10.1007/s11033-020-05409-3
Bari V.K., Nassar J.A., Kheredin S.M., Gal-On A., Ron M., Britt A., Steele D., Yoder J., Aly R. (2019) CRISPR/Cas9-mediated mutagenesis of carotenoid cleavage dioxygenase 8 in tomato provides resistance against the parasitic weed Phelipanche aegyptiaca. Sci. Rep. 9, 11438. https://doi.org/10.1038/s41598-019-47893-z
Ortigosa A., Gimenez-Ibanez S., Leonhardt N., Solano R. (2019) Design of a bacterial speck resistant tomato by CRISPR/Cas9-mediated editing of SlJAZ2. Plant Biotechnol. J. 17(3), 665–673.https://doi.org/10.1111/pbi.13006
Li X., Wang Y., Chen S., Tian H., Fu D., Zhu B., Luo Y., Zhu H. (2018) Lycopene is enriched in tomato fruit by CRISPR/Cas9-mediated multiplex genome editing. Front. Plant Sci. 9, 559. https://doi.org/10.3389/fpls.2018.00559
Ahmar S., Zhai Y., Huang H., Yu K., Hafeez M., Khan U., Shahid M., Samad R.A., Khan S.U., Amoo O., Fan C., Zhou Y. (2020) Development of mutants with varying flowering times by targeted editing of multiple SVP gene copies in Brassica napus L. Crop J. 10(1), 67–74. https://doi.org/10.1016/j.cj.2021.03.023
Cao Y., Yan X., Ran S., Ralph J., Smith R.A., Chen X., Qu C., Li J., Liu L. (2022) Knockout of the lignin pathway gene BnF5H decreases the S/G lignin compositional ratio and improves Sclerotinia sclerotiorum resistance in Brassica napus. Plant Cell Environ. 45(1), 248‒261. https://doi.org/10.1111/pce.14208
Fan S., Zhang L., Tang M., Cai Y., Liu J., Liu H., Liu J., Terzaghi W., Wang H., Hua W., Zheng M. (2021) CRISPR/Cas9-targeted mutagenesis of the BnaA03.BP gene confers semi-dwarf and compact architecture to rapeseed (Brassica napus L.). Plant Biotechnol. J. 19(12), 2383–2385. https://doi.org/10.1111/pbi.13703
Zhang X., Cheng J., Lin Y., Fu Y., Xie J., Li B., Bian X., Feng Y., Liang W., Tang Q., Zhang H., Liu X., Zhang Y., Liu C., Jiang D. (2021) Editing homologous copies of an essential gene affords crop resistance against two cosmopolitan necrotrophic pathogens. Plant Biotechnol. J. 19(11), 2349–2361. https://doi.org/10.1111/pbi.13667
Wang Z., Wan L., Xin Q., Zhang X., Song Y., Wang P., Hong D., Fan Z., Yang G. (2021) Optimizing glyphosate tolerance in rapeseed by CRISPR/Cas9-based geminiviral donor DNA replicon system with Csy4-based single-guide RNA processing. J. Exp. Bot. 72(13), 4796–4808. https://doi.org/10.1093/jxb/erab167
Zaman Q.U., Wen C., Yuqin S., Mengyu H., Desheng M., Jacqueline B., Baohong Z., Chao L., Qiong H. (2021) Characterization of SHATTERPROOF homoeologs and CRISPR-Cas9-mediated genome editing enhances pod-shattering resistance in Brassica napus L. CRISPR J. 4(3), 360–370. https://doi.org/10.1089/crispr.2020.0129
Probsting M., Schenke D., Hossain R., Thurau C., Wighardt L., Schuster A., Zhou Z., Ye W., Rietz S., Leckband G., Cai D. (2020) Loss-of-function of CRT1a (calreticulin) reduces susceptibility to Verticillium longisporum in both Arabidopsis thaliana and oilseed rape (Brassica napus). Plant Biotechnol. J. 18, 2328–2344. https://doi.org/10.1111/pbi.13394
Sashidhar N., Harloff H.J., Potgieter L., Jung C. (2020) Gene editing of three BnITPK genes in tetraploid oilseed rape leads to significant reduction of phytic acid in seeds. Plant Biotechnol. J. 18(11), 2241‒2250. https://doi.org/10.1111/pbi.13380
Karunarathna N.L., Wang H., Harloff H.-J., Jiang L., Jung C. (2020) Elevating seed oil content in a polyploid crop by induced mutations in SEED FATTY ACID REDUCER genes. Plant Biotechnol. J. 18(11), 2251–2266. https://doi.org/10.1111/pbi.13381
Wu J., Chen C., Xian G., Liu D., Lin L., Yin S., Sun Q., Fang Y., Zhang H., Wang Y. (2020) Engineering herbicide-resistant oilseed rape by CRISPR/Cas9-mediated cytosine base-editing. Plant Biotechnol. J. 18(9), 1857–1859.https://doi.org/10.1111/pbi.13368
Xie T., Chen X., Guo T., Rong H., Chen Z., Sun Q., Batley J., Jiang J., Wang Y. (2020) Targeted knockout of BnTT2 homologues for yellow-seeded Brassica napus with reduced flavonoids and improved fatty acid composition. J. Agric. Food Chem. 68(20), 5676–5690.https://doi.org/10.1021/acs.jafc.0c01126
Wu J., Yan G., Duan Z., Wang Z., Kang C., Guo L., Liu K., Tu J., Shen J., Yi B., Fu T., Li X., Ma C., Dai C. (2020) Roles of the Brassica napus DELLA protein BnaA6.RGA, in modulating drought tolerance by interacting with the ABA signaling component BnaA10.ABF2, Front. Plant Sci. 11. 577. https://doi.org/10.3389/fpls.2020.00577
Zhai Y., Yu K., Cai S., Hu L., Amoo O., Xu L., Yang Y., Ma B., Jiao Y., Zhang C., Khan M.H.U., Khan S.U. (2020) Targeted mutagenesis of BnTT8 homologs controls yellow seed coat development for effective oil production in Brassica napus L. Plant Biotechnol. J. 18(5), 1153–1168. https://doi.org/10.1111/pbi.13281
Zheng M., Zhang L., Tang M., Liu J., Liu H., Yang H., Fan S., Terzaghi, W., Wang H., Hua W. (2020) Knockout of two Bna MAX 1 homologs by CRISPR/Cas9-targeted mutagenesis improves plant architecture and increases yield in rapeseed (Brassica napus L.). Plant Biotechnol. J. 18(3), 644–654. https://doi.org/10.1111/pbi.13228
Khan M.H.U., Hu L., Zhu M., Zhai Y., Khan S.U., Ahmar S., Amoo O., Zhang K., Fan C., Zhou Y. (2021) Targeted mutagenesis of EOD3 gene in Brassica napus L. regulates seed production. J. Cell. Physiol. 236(3), 1996–2007.https://doi.org/10.1002/jcp.29986
Sun Q., Lin L., Liu D., Wu D., Fang Y., Wu J., Wang Y. (2018) CRISPR/Cas9-mediated multiplex genome editing of the BnWRKY11 and BnWRKY70 genes in Brassica napus L. Int. J. Mol. Sci. 19(9), 2716. https://doi.org/10.3390/ijms19092716
Kim Y.C., Ahn W.S., Cha A., Jie E. Y., Kim S. W., Hwang B‑H., Lee S. (2022) Development of glucoraphanin-rich broccoli (Brassica oleracea var. italica) by CRISPR/Cas9-mediated DNA-free BolMYB28 editing. Plant Biotechnol. Rep. 16, 123–132. https://doi.org/10.1007/s11816-021-00732-y
Jeong S.Y., Ahn H., Ryu J., Oh Y., Sivanandhan G., Won K.‑H., Park Y.D., Kim J.‑S., Kim H., Lim Y.P., Kim S.‑G. (2019) Generation of early-flowering chinese cabbage (Brassica rapa spp. pekinensis) through CRISPR/Cas9-mediated genome editing. Plant Biotechnol. Rep. 13(5), 491–499. https://doi.org/10.1007/s11816-019-00566-9
Neequaye M., Stavnstrup S., Harwood W., Lawrenson T., Hundleby P., Irwin J., Troncoso-Rey P., Saha S., Traka M.H., Mithen R., Østergaard L. (2021) CRISPR-Cas9-mediated gene editing of MYB28 genes impair glucoraphanin accumulation of Brassica oleracea in the field. CRISPR J. 4(3), 416–426. https://doi.org/10.1089/crispr.2021.0007
Cao W., Dong X., Ji J., Yang L., Fang Z., Zhuang M., Zhang Y., Lv H., Wang Y., Sun P., Liu Y., Li Z., Han F. (2021) BoCER1 is essential for the synthesis of cuticular wax in cabbage (Brassica oleracea L. var. capitata). Scientia Horticulturae. 277, 109801. https://doi.org/10.1016/j.scienta.2020.109801
Mishra R., Mohanty J.N., Mahanty B., Joshi R.K. (2021) A single transcript CRISPR/Cas9 mediated mutagenesis of CaERF28 confers anthracnose resistance in chilli pepper (Capsicum annuum L.). Planta. 254(1), 5. https://doi.org/10.1007/s00425-021-03660-x
Lee K.-R., Jeon I., Yu H., Kim S.-G., Kim H.-S., Ahn S.-J., Lee J., Lee S.-K., Kim H.U. (2021) Increasing monounsaturated fatty acid contents in hexaploid Camelina sativa seed oil by FAD2 gene knockout using CRISPR-Cas9. Front. Plant Sci. 12, 702930. https://doi.org/10.3389/fpls.2021.702930
Janga M.R., Pandeya D., Campbell L.M., Konganti K., Villafuerte S.T., Puckhaber L., Pepper A., Stipanovic R.D., Scheffler J.A, Rathore K.S. (2019). Genes regulating gland development in the cotton plant. Plant Biotechnol. J. 17(6), 1142–1153. https://doi.org/10.1111/pbi.13044
Chen Y., Fu M., Li H., Wang L., Liu R., Liu Z., Zhang X., Jin S. (2021) High-oleic acid content, n-ontransgenic allotetraploid cotton (Gossypium hirsutum L.) generated by knockout of GhFAD2 genes with CRISPR/Cas9 system. Plant Biotechnol. J. 19(3), 424–426. https://doi.org/10.1111/pbi.13507
Вавилов Н.И. (1920) Закон гомологических рядов в наследственной изменчивости. Доклад на 3-й Всероссийской встрече селекционеров. Саратов, с. 16.
Li Z., Liu Z. B., Xing A., Moon B.P., Koellhoffer J.P., Huang L., Cigan A.M. (2015). Cas9-guide RNA directed genome editing in soybean. Plant Physiol. 169(2), 960—970.
Butt H., Eid A., Ali Z., Atia M.A., Mokhtar M.M., Hassan N., Mahfouz M.M. (2017) Efficient CRISPR/-Cas9-mediated genome editing using a chimeric single-guide RNA molecule. Front. Plant Sci. 8, 1441.
Shimatani Z., Kashojiya S., Takayama M., Terada R., Arazoe T., Ishii H., Teramura H., Yamamoto T., Komatsu H., Miura K., Ezura H., Nishida K., Ariizumi T., Kondo A. (2017) Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat. Biotechnol. 35, 441–443. https://doi.org/10.1038/nbt.3833
Shimatani Z., Fujikura U., Ishii H., Matsui Y., Suzuki M., Ueke Y., Taoka K., Terada R., Nishida K., Kondo A. (2018) Inheritance of co-edited genes by CRISPR-based targeted nucleotide substitutions in rice. Plant Physiol. Biochem. 131, 78‒83. https://doi.org/. 2018.04.028https://doi.org/10.1016/J.PLAPHY
Shimatani Z., Fujikura U., Ishii H., Terada R., Nishida K., Kondo A. (2018) Herbicide tolerance-assisted multiplex targeted nucleotide substitution in rice. Data Brief. 20, 1325‒1331. https://doi.org/10.1016/J.DIB.2018.08.124
Sun Y., Jiao G., Liu Z., Zhang X., Li J., Guo X., Du W., Du J., Francis F., Zhao Y., Xia L. (2017) Generation of high-amylose rice through CRISPR/Cas9-mediated targeted mutagenesis of starch branching enzymes. Front. Plant Sci. 8, 298. https://doi.org/10.3389/fpls.2017.00298
Butler N.M., Baltes N.J., Voytas D.F., Douches D.S. (2016) Geminivirus-mediated genome editing in potato (Solanum tuberosum L.) using sequence-specific nucleases. Front. Plant Sci. 7, 1045. https://doi.org/10.3389/fpls.2016.01045
Svitashev S., Young J.K., Schwartz C., Gao H., Falco S.C., Cigan A.M. (2015) Targeted mutagenesis, precise gene editing, and site-specific gene insertion in maize using Cas9 and guide RNA. Plant Physiol. 169(2), 931–945. https://doi.org/10.1104/pp.15.00793
Braatz J., Harloff H.J., Mascher M., Stein N., Himmelbach A., Jung C. (2017) CRISPR-Cas9 targeted mutagenesis leads to simultaneous modification of different homoeologous gene copies in polyploid oilseed rape (Brassica napus). Plant Physiol. 174(2), 935‒942. https://doi.org/10.1104/pp.17.00426
Liu X., Ding Q., Wang W., Pan Y., Tan C., Qiu Y., Chen Y., Li H., Li Y., Ye N., Xu N., Wu X., Ye R., Liu J., Ma C. (2022) Targeted deletion of the first intron of the Wxb allele via CRISPR/Cas9 significantly increases grain amylose content in rice. Rice (N.Y.). 15(1), 1. https://doi.org/10.1186/s12284-021-00548-y
Yunyan F., Jie Y., Fangquan W., Fangjun F., Wenqi L., Jun W., Yang X., Jinyan Z., Weigong Z. (2019) Production of two elite glutinous rice varieties by editing Wx gene. Rice Sci. 26, 118‒124. https://doi.org/10.1016/j.rsci.2018.04.007
Zhang J., Zhang H., Botella J.R., Zhu J.K. (2018) Generation of new glutinous rice by CRISPR/Cas9-targeted mutagenesis of the Waxy gene in elite rice varieties. J. Integr. Plant Biol. 60(5), 369‒375. https://doi.org/10.1111/jipb.12620
Veillet F., Chauvin L., Kermarrec M.P., Sevestre F., Chauvin J.E. (2019) The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato. Plant Cell Rep. 38, 1065–1080. https://doi.org/10.1007/s00299-019-02426-w
Kusano H., Ohnuma M., Mutsuro-Aoki H., Asahi T., Ichinosawa D., Onodera H., Asano K., Noda T., Horie T., Fukumoto K., Kihira M., Teramura H., Yazaki K., Umemoto N., Muranaka T., Shimada H. (2018) Establishment of a modified CRISPR/Cas9 system with increased mutagenesis frequency using the translational enhancer dMac3 and multiple guide RNAs in potato. Sci. Rep. 8, 13753. https://doi.org/10.1038/s41598-018-32049-2
Andersson M., Turesson H., Nicolia A., Fält A.S., Samuelsson M., Hofvander P. (2017) Efficient targeted multiallelic mutagenesis in tetraploid potato (Solanum tuberosum) by transient CRISPR-Cas9 expression in protoplasts. Plant Cell Rep. 36(1), 117‒128. https://doi.org/10.1007/s00299-016-2062-3
Qi X., Wu H., Jiang H., Zhu J., Huang C., Zhang X., Liu C., Cheng B. (2020) Conversion of a normal maize hybrid into a waxy version using in vivo CRISPR/Cas9 targeted mutation activity. Crop J. 8, 440‒448. https://doi.org/10.1016/j.cj.2020.01.006
Abe K., Araki E., Suzuki Y., Toki S., Saika H. (2018) Production of high oleic/low linoleic rice by genome editing. Plant Physiol. Biochem. 131, 58‒62. https://doi.org/10.1016/J.PLAPHY.2018.04.033
Okuzaki A., Ogawa T., Koizuka C., Kaneko K., Inaba M., Imamura J., Koizuka N. (2018) CRISPR/Cas9-mediated genome editing of the fatty acid desaturase 2 gene in Brassica napus. Plant Physiol. Biochem. 131, 63‒69. https://doi.org/10.1016/J.PLAPHY.2018.04.025
Chandrasekaran J., Brumin M., Wolf D., Leibman D., Klap C., Pearlsman M., Gal-On A. (2016) Development of broad virus resistance in non-transgenic cucumber using CRISPR/Cas9 technology. Mol. Plant Pathol. 17(7), 1140‒1153. https://doi.org/10.1111/mpp.12375
Jia H., Zhang Y., Orbović V., Xu J., White F.F., Jones J.B., Wang N. (2017) Genome editing of the disease susceptibility gene CsLOB1 in citrus confers resistance to citrus canker. Plant Biotechnol. J. 15(7), 817‒823. https://doi.org/10.1111/pbi.12677
Peng A., Chen S., Lei T., Xu L., He Y., Wu L., Yao L., Zou X. (2017) Engineering canker-resistant plants through CRISPR/Cas9-targeted editing of the susceptibility gene CsLOB1 promoter in citrus. Plant Biotechnol. J. 15(12), 1509‒1519. https://doi.org/10.1111/pbi.12733
Malnoy M., Viola R., Jung M.H., Koo O.J., Kim S., Kim J.S., Nagamangala Kanchiswamy C. (2016) DNA-free genetically edited grapevine and apple protoplast using CRISPR/Cas9 ribonucleoproteins. Front. Plant Sci. 7, 1904.
Nekrasov V., Wang C., Win J., Lanz C., Weigel D., Kamoun S. (2017) Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion. Sci. Rep. 7(1), 1‒6. https://doi.org/10.1038/s41598017-00578-x
Wang Y., Cheng X., Shan Q., Zhang Y., Liu J., Gao C., Qiu J.L. (2014) Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew. Nat. Biotechnol. 32(9), 947‒951. https://doi.org/10.1038/nbt.2969
Zhang Y., Bai Y., Wu G., Zou S., Chen Y., Gao C., Tang D. (2017) Simultaneous modification of three homoeologs of TaEDR1 by genome editing enhances powdery mildew resistance in wheat. Plant J. 91(4), 714‒724. https://doi.org/10.1111/tpj.13599
Hu X., Cui Y., Dong G., Feng A., Wang D., Zhao C., Zhang Y., Hu J., Zeng D., Guo L., Qian Q. (2019) Using CRISPR-Cas9 to generate semi-dwarf rice lines in elite landraces. Sci. Rep. 9, 19096. https://doi.org/10.1038/s41598-019-55757-9
Khlestkina E.K., Shvachko N.A., Zavarzin A.A., Börner A. (2020) Vavilov′s series of the “green revolution” genes. Russ. J. Genetics. 56(11), 1371‒1380. https://doi.org/10.1134/S1022795420110046
Zhang S., Zhang R., Song G., Gao J., Li W., Han X., Chen M., Li Y., Li G. (2018) Targeted mutagenesis using the Agrobacterium tumefaciens-mediated CRISPR-Cas9 system in common wheat. BMC Plant Biol. 18, 302. https://doi.org/10.1186/s12870-018-1496-x
Kim C.Y., Park J.Y., Choi G., Kim S., Vo K.T.X., Jeon J.S., Lee Y.H. (2022) A rice gene encoding glycosyl hydrolase plays contrasting roles in immunity depending on the type of pathogens. Mol. Plant Pathol. 23(3), 400‒416. https://doi.org/10.1111/mpp.13167
Carey-Fung O., O’Brien M., Beasley J.T., Johnson A.A.T. (2022) A model to incorporate the bHLH transcription factor OsIRO3 within the rice iron homeostasis regulatory network. Int. J. Mol. Sci. 23, 1635. https://doi.org/10.3390/ijms23031635
Takeda Y., Tobimatsu Y., Karlen S.D., Koshiba T., Suzuki S., Yamamura M., Murakami S., Mukai M., Hattori T., Osakabe K., Ralph J., Sakamoto M., Umezawa T. (2018) Downregulation of p-COUMAROYL ESTER 3-HYDROXYLASE in rice leads to altered cell wall structures and improves biomass saccharification. Plant J. 95(5), 796‒811. https://doi.org/10.1111/tpj.13988
Дополнительные материалы отсутствуют.
Инструменты
Молекулярная биология