Молекулярная биология, 2023, T. 57, № 4, стр. 587-596

Альтернативные механизмы мутагенеза в mCpG-сайтах при репликации и репарации

Е. С. Шилкин a, Д. В. Петрова bc, Д. О. Жарков bc*, А. В. Макарова a**

a Институт молекулярной генетики, Национальный исследовательский центр “Курчатовский институт”
123182 Москва, Россия

b Институт химической биологии и фундаментальной медицины Сибирского отделения Российской академии наук
630090 Новосибирск, Россия

c Новосибирский государственный университет
630090 Новосибирск, Россия

* E-mail: dzharkov@niboch.nsc.ru
** E-mail: amakarova-img@yandex.ru

Поступила в редакцию 08.12.2022
После доработки 06.02.2023
Принята к публикации 08.02.2023

Аннотация

5-Метил-2'-дезоксицитидин (mC) в составе СpG-сайтов играет ключевую роль в эпигенетической регуляции работы генов, механизмах дифференцировки клеток и канцерогенезе. Несмотря на важность mС для нормального функционирования клетки, CpG-динуклеотиды являются горячими точками мутагенеза. mC дезаминируется с образованием Т, вызывая транзиции C→T. Однако в последние годы появляются отдельные исследования о влиянии эпигенетических модификаций С на точность и эффективность работы ДНК-полимераз и ферментов эксцизионной репарации. В настоящем обзоре обобщены данные, указывающие на существование независимых от дезаминирования механизмов мутагенеза в CpG-сайтах.

Ключевые слова: 5-метил-2'-дезоксицитидин, CpG-сайты, ДНК-полимеразы, повреждения ДНК, репарация

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