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Молекулярная биология, 2023, T. 57, № 5, стр. 833-852

Продолжительность жизни Drosophila melanogaster регулируется экспрессией гена nejire в периферических тканях и нервной системе

Л. А. Коваль a, Е. Н. Прошкина a, Н. В. Земская a, И. А. Соловьёв a, b, Е. В. Щеголева a, М. В. Шапошников a, А. А. Москалев a, c, d, *

a Институт биологии Коми научного центра Уральского отделения Российской академии наук
167982 Сыктывкар, Россия

b Сыктывкарский государственный университет им. Питирима Сорокина
167001 Сыктывкар, Россия

c Институт молекулярной биологии им. В.А. Энгельгардта Российской академии наук
119991 Москва, Россия

d Российский клинический научный центр геронтологии, Российский национальный исследовательский медицинский университет им. Н.И. Пирогова Министерства здравоохранения Российской Федерации
129226 Москва, Россия

* E-mail: amoskalev@ib.komisc.ru

Поступила в редакцию 25.12.2022
После доработки 27.03.2023
Принята к публикации 28.03.2023

Аннотация

Гистонацетилтрансферазы семейства CBP/p300 участвуют в регуляции транскрипции и в осуществлении ряда биологических процессов (пролиферации и дифференцировки клеток, развитии организма, регуляции стресс-ответа и метаболизма). В исследовании на плодовой мушке Drosophila melanogaster нами впервые проанализировано влияние сверхэкспрессии и нокдауна гена nejire (nej), кодирующего ортолог белков CBP/p300 человека, в различных тканях (жировом теле, кишечнике, нервной системе) и на разных стадиях жизненного цикла (все стадии развития или только имаго) на продолжительность жизни. Активация nej оказывала, в зависимости от способа индукции, а также от половой принадлежности, как положительное, так и отрицательное влияние на продолжительность жизни мух. Установлен эффект увеличения продолжительности жизни (на 6–15%) самок при кондиционной сверхэкспрессии nej в кишечнике и конститутивной сверхэкспрессии nej в нервной системе. В остальных случаях наблюдали укорочение жизни (до 44%) либо отсутствие статистически значимых изменений. Кроме того, активация nej приводила к изменению экспрессии генов стресс-ответа (Sod1, Gadd45, Hsp27, Hsp68, Hif1). В то же время нокдаун nej в большинстве вариантов эксперимента приводил к выраженному отрицательному воздействию на длительность жизни дрозофил.

Ключевые слова: гистонацетилтрансфераза, CBP/p300, nejire, продолжительность жизни, стрессоустойчивость, Drosophila melanogaster

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Приложение 1.
Таблица S1. Влияние сверхэкспрессии гена nej в различных тканях на выживаемость дрозофил в неблагоприятных условиях

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