Журнал эволюционной биохимии и физиологии, 2020, T. 56, № 7, стр. 733-733

The Effect of Metformin Treatment on the Baseline and Gonadotropin-Stimulated Steroidogenesis in Male Wistar Rats with Severe Type 2 Diabetes

A. A. Bakhtyukov 1*, K. V. Derkach 1, I. V. Romanova 1, I. I. Zorina 1, L. V. Bayunova 1, V. M. Bondareva 1, I. Yu. Morina 1, L. Annie 2, M. Jeremy 2, G. Gurusubramanian 2, V. K. Roy 2, A. O. Shpakov 1

1 Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences
Saint-Petersburg, Russia

2 Mizoram University
Mizoram, Aizawl, India

* E-mail: bahtyukov@gmail.ru

Полный текст (PDF)

Type 2 diabetes mellitus impairs the reproductive functions in men, and important tasks are deciphering the molecular mechanisms of testicular dysfunctions in diabetes and the search of effective approaches to their correction. The purpose was to study the regulatory effects of four-week metformin treatment (120 mg/kg/day) of male Wistar rats with high-fat diet/low-dose streptozotocin-induced type 2 diabetes on the basal and gonadotropin-stimulated steroidogenesis, the intratesticular content of leptin and the receptors of leptin and luteinizing hormone/chorionic gonadotropin and on the sperm quality. Diabetic rats had hyperleptinemia, androgen deficiency, and reduced sperm number and motility, and in the diabetic testes, they had the increased leptin content and the reduced content of the leptin and luteinizing hormone/chorionic gonadotropin receptors and 17-hydroxyprogesterone. The stimulating effects of human chorionic gonadotropin on testosterone production and expression of steroidogenic genes (Star, Cyp11a1) were decreased. Metformin restored the basal and gonadotropin-stimulated plasma testosterone levels. In the testes, it restored gonadotropin-stimulated 17-hydroxyprogesterone, androstenedione and testosterone levels, the Star expression and the levels of leptin and the leptin and luteinizing hormone/chorionic gonadotropin receptors. Metformin also improved quantity and quality of sperm and morphology of the convoluted seminiferous tubules. We concluded that metformin treatment normalizes the testicular steroidogenesis in diabetic rats, which is due to restoration of the gonadotropin and leptin systems in the testes and is associated with an improvement in spermatogenesis.

Supported by RFBR 18-515-45004-IND-a; DST INT/RUS/RFBR/P-310.

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