Журнал эволюционной биохимии и физиологии, 2020, T. 56, № 7, стр. 769-769

Comparative Analysis of Expression of Some Protein Kinases and Protein Phosphatases in Lamprey Erythrocytes on the Course of Prespawning Period

I. A. Khvorova 12*, N. I. Agalakova 1

1 Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences
Saint-Petersburg, Russia

2 FGBOU VO Saint Petersburg State University of Veterinary Medicine
Saint-Petersburg, Russia

* E-mail: ira99-2012@mail.ru

Полный текст (PDF)

The living cycle of lamprey Lampetra fluviatilis is terminated by prespawning period of genetically determined starvation accompanied by development of metabolic depression. This period is interesting for both studying the mechanisms of adaptation to the state which is pathological for the majority of organisms and for the strategies of defense allowing the animals to survive. One of such mechanisms is a coordinated functioning of intracellular enzymes, above all protein kinases and protein phosphatases.

The goal of work was identification and comparative analysis of expression of some MAP kinases and tyrosine phosphatases in lamprey erythrocytes on the course of prespawning period. The content of enzymes was evaluated by immunoblotting in the cytosolic and membrane fractions of erythrocytes.

At the beginning of prespawning period (in December) ERK1/2 kinase is found in both cytosol and membranes of erythrocytes, whereas JNK – in the cytosol only. Phosphatases PTP1B and SHPTP2 are revealed in both subcellular fractions, with РТР1В being presenting by native and proteolytic fragments. The expression of ERK1/2 is relatively stable on the course of entire period of starvation, although before spawning its content decreased a little in the cytosol, but increased in membranes on account of active phosphorylated form. The activity of JNK in erythrocytes, in contrast, sharply falls before spawning, with phosphor-JNK virtually not revealing. Total content of PTP1B is relatively constant during prespawning period, however, at the spring an expression of the native form of enzyme decreases in membranes, but the content of proteolytic fragment increases in the cytosol. An activity of SHPTP2 phosphatase does not change on the course of prespawning starvation.

Most probably, the membrane-bound forms of enzymes are necessary for the maintenance of viability of lamprey erythrocytes, especially before spawning and death of the animals in May, for example, for the preservation of integrity of plasma membrane and regulation of transmembrane ion transport.

Supported by state budget (АААА-А18-118012290371-3).

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