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  4. T. 57, № 3, 2023

Молекулярная биология, 2023, T. 57, № 3, стр. 440-457

Методы обогащения ДНК-фрагментов для высокопроизводительного секвенирования

А. Н. Синяков a, *, Е. В. Костина a

a Институт химической биологии и фундаментальной медицины, Сибирское отделение Российской академии наук
630090 Новосибирск, Россия

* E-mail: sinyakov@niboch.nsc.ru

Поступила в редакцию 08.11.2022
После доработки 16.12.2022
Принята к публикации 16.12.2022

Аннотация

В обзоре описано использование олигонуклеотидов, в основном полученных с помощью синтезаторов ДНК нового поколения (микрочиповых синтезаторов ДНК), для обогащения целевых геномных фрагментов. Рассмотрены способы обогащения целевых мишеней с использованием методов молекулярной гибридизации, ПЦР и системы CRISPR-Cas9. Приведены примеры практического применения разработанных методов для исследовательских и диагностических целей.

Ключевые слова: высокопроизводительное секвенирование, олигонуклеотиды, микрочипы, амплификация, гибридизация, диагностика, экзом, CRISPR-Cas9

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