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  4. T. 57, № 4, 2023

Молекулярная биология, 2023, T. 57, № 4, стр. 668-670

Комбинированная сверхэкспрессия Foxa3 и Hnf4a усиливает пролиферацию и продлевает функциональную сохранность первичных гепатоцитов

J. Y. Fan a, b, c, G. Dama a, d, Y. L. Liu a, b, W. Y. Guo b, J. T. Lin a, b, e, *

a Stem Cell and Biotherapy Engineering Research Center of Henan, Henan Joint International Research Laboratory of Stem Cell Medicine, Xinxiang Medical University
453003 Xinxiang, China

b College of Life Science and Technology, Xinxiang Medical University, Henan
453003 Xinxiang, China

c Shandong Tianchuan Precision Medical Technology Co. Ltd.
253084 Dezhou, China

d Department of Community Health, Advanced Medical and Dental Institute, University Sains Malaysia
13200 Kepala Batas, Malaysia

e College of Biomedical Engineering, Xinxiang Medical University, Henan
453003 Xinxiang, China

* E-mail: linjtlin@126.com

Поступила в редакцию 05.09.2022
После доработки 07.11.2022
Принята к публикации 06.12.2022

Аннотация

В системе культивирования in vitro пролиферативная активность первичных гепатоцитов обычно низкая и сопровождается снижением жизнеспособности и потерей специфических для гепатоцитов функций. Ранее показано, что комбинированное введение определенных гепатоцитспецифических факторов транскрипции приводит к конвертированию фибробластов в функциональные гепатоцитоподобные клетки. Однако комбинированное использование факторов транскрипции в первичной культуре гепатоцитов еще недостаточно изучено. Белки FoxA3 (forkhead box protein А3) и Hnf4α (forkhead box protein А3 hepatocyte nuclear factor 4α) представляют собой факторы транскрипции, которыми обогащена печень, и играют жизненно важную роль в дифференцировке и поддержании гепатоцитов. В представленном исследовании в гепатоцитах крысы получили одновременную избыточную экспрессию двух генов: Foxa3 и Hnf4a. Показано, что комбинированное усиление экспрессии двух транскрипционных факторов: FoxA3 и Hnf4α ‒ приводит к повышенной скорости пролиферации и стабилизации специфических функций первичных гепатоцитов в течение длительного периода культивирования.

Ключевые слова: гепатоциты, пролиферация, печень, FoxA3, Hnf4α

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