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Российский физиологический журнал им. И.М. Сеченова, 2023, T. 109, № 7, стр. 819-843

GCaMP – семейство однофлуорофорных генетически-кодируемых кальциевых индикаторов

А. И. Ерофеев 1, *, Е. К. Винокуров 1, О. Л. Власова 1, И. Б. Безпрозванный 1, 2

1 Лаборатория молекулярной нейродегенерации, Институт биомедицинских систем и биотехнологий, Санкт-Петербургский Политехнический университет Петра Великого
Санкт-Петербург, Россия

2 Отделение физиологии, Юго-Западный медицинский центр Техасского университета, Даллас
Техас, США

* E-mail: alexandr.erofeew@gmail.com

Поступила в редакцию 04.05.2023
После доработки 16.06.2023
Принята к публикации 17.06.2023

Аннотация

Однофлуорофорные генетически кодируемые кальциевые индикаторы, такие как GCaMP, являются широко используемыми инструментами для изучения нейрональной активности. Их главным преимуществом является способность в режиме реального времени и с высокой чувствительностью реагировать на изменения концентрации ионов кальция в клетке. Это особенно важно для исследования нейрональных процессов и нейронных ансамблей, где кальциевые сигналы играют ключевую роль в передаче информации. Данный обзор рассматривает семейство кальциевых индикаторов GCaMP, описывает их разновидности, особенности и перспективы использования для визуализации нейронной активности. Особое внимание уделяется тенденции совершенствования GCaMP, в частности направленной на расширение их спектральных свойств и способности регистрировать высокочастотную спайковую активность.

Ключевые слова: кальциевые индикаторы, нейронная активность, динамика, флуоресценция

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