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Коллоидный журнал, 2023, T. 85, № 5, стр. 655-667

Концентрация наночастиц как важный параметр для характеристики дисперсий и ее применение в биомедицине

Т. Н. Паширова 1, *, З. М. Шайхутдинова 1, Э. Б. Соуто 2, 3, П. Массон 4, В. Ф. Миронов 1

1 Институт органической и физической химии им. А.Е. Арбузова, ФИЦ Казанский научный центр РАН
420088 Казань, ул. Акад. Арбузова, 8, Россия

2 UCIBIO – Applied Molecular Biosciences Unit, MEDTECH, Laboratory of Pharmaceutical Technology, Department of Drug Sciences, Faculty of Pharmacy, University of Porto
4050-313 Porto, Portugal

3 Associate Laboratory i4HB – Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto
4050-313 Porto, Portugal

4 Казанский (Приволжский) федеральный университет
420008 Казань, ул. Кремлевская, 18, Россия

* E-mail: tatyana_pashirova@mail.ru

Поступила в редакцию 26.06.2023
После доработки 03.08.2023
Принята к публикации 03.08.2023

Аннотация

В настоящее время стоит острая необходимость в стандартизации методов определения концентрации наночастиц и создания эталонных материалов для калибровки измеряемой величины. Точное определение концентрации наночастиц необходимо для оценки максимальной дозы вводимых нанопрепаратов в области диагностики и терапии in vivo, определения порядка реакции при использовании ферментативных нанореакторов. Кроме того, данный параметр обуславливает биологические эффекты, такие как образование белковой короны, улучшение поглощения и интернализации с клетками и т.д. В обзоре представлены наиболее часто встречающиеся способы определения концентрации наночастиц, основанные на их прямой визуализации с использованием методов микроскопии, на поглощении или рассеянии света, прямого подсчета наночастиц и гравиметрии, обсуждены их достоинства, недостатки и способы усовершенствования. Показано, что для более надежного и достоверного определения концентрации наночастиц следует использовать комбинацию нескольких методов.

Ключевые слова: система доставки лекарств, концентрация наночастиц, наночастица, доза-эффект, клеточное поглощение, интернализация, нанореактор

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