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  4. T. 39, № 3, 2022

Биологические мембраны: Журнал мембранной и клеточной биологии, 2022, T. 39, № 3, стр. 172-185

Принципы и проблемы выделения экзосом из биологических жидкостей

Е. И. Якубович a, *, А. Г. Полищук a, В. И. Евтушенко a

a Российский научный центр радиологии и хирургических технологий им. академика А. М. Гранова Минздрава России
197758 Санкт-Петербург, Россия

* E-mail: jakubovichelena@mail.ru

Поступила в редакцию 14.01.2022
После доработки 20.01.2022
Принята к публикации 21.01.2022

Аннотация

Экзосомы, подкласс малых мембранных внеклеточных везикул, имеют большой диагностический и терапевтический потенциал, однако отсутствие стандартизированных методов их эффективного выделения и анализа ограничивает внедрение экзосомальных технологий в клиническую практику. В представленном обзоре рассматриваются проблемы, связанные с выделением экзосом из биологических жидкостей, и принципы традиционных и альтернативных методов выделения. Цель представленного обзора – проиллюстрировать разнообразие подходов, основанных на физических и биохимических свойствах экзосом, которые могут быть использованы для выделения экзосом. Обсуждаются достоинства и недостатки разных методов.

Ключевые слова: микровезикулы, экзосомы, биосепарация, методы выделения

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