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Цитология, 2023, T. 65, № 6, стр. 583-592

Белок щелевых контактов коннексин-43 в глиальных клетках чувствительного ганглия крысы

Е. А. Колос 1, *, Д. Э. Коржевский 1

1 Институт экспериментальной медицины
197376 Санкт-Петербург, Россия

* E-mail: koloselena1984@yandex.ru

Поступила в редакцию 19.06.2023
После доработки 28.07.2023
Принята к публикации 31.07.2023

Аннотация

Цель настоящего исследования состояла в изучении динамики распределения и локализации белка щелевых контактов коннексина-43 (Сx43) в клетках ганглия заднего корешка спинного мозга (dorsal root ganglion, DRG) крысы на разных этапах постнатального онтогенеза для оценки морфологических признаков возрастных изменений межклеточных взаимодействий. Работа выполнена на крысах Вистар в возрасте 4 и 18 мес с помощью иммуногистохимических методов. Глиальные клетки выявляли с применением антител к глутаминсинтетазе, макрофаги – с применением маркера Iba-1. Установлено, что коннексин-43-содержащие структуры идентифицируются преимущественно в сателлитных глиальных клетках молодых и стареющих животных. Чувствительные нейроны, а также макрофаги DRG крыс исследованных возрастных групп коннексин-43 не содержат. При анализе возрастных изменений межклеточных контактов в DRG крыс было установлено, что зоны, обогащенные коннексином-43, соответствующие бляшкам (plaques) белковых каналов, обеспечивающие метаболическое взаимодействие сателлитных клеток в ганглиях задних корешков спинного мозга, с возрастом становятся более многочисленными. Данный факт может свидетельствовать об активации взаимодействия между глиальными клетками в чувствительных узлах крыс при старении.

Ключевые слова: старение, ганглий заднего корешка спинного мозга, иммуногистохимия, белок Iba-1, коннексин-43, щелевые каналы, межклеточные взаимодействия

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